International Journal of Radiation Research

en Potential molecular mechanisms of Huangqi in radiotherapy-induced kidney injury associated with chronic kidney disease: A network pharmacology study Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Radiotherapy is an important treatment for cancer but may cause renal injury, particularly in patients with pre-existing or treatment-related chronic kidney disease (CKD). Huangqi (Astragalus membranaceus) has been widely used in traditional Chinese medicine for kidney-related disorders. This study explored the potential molecular mechanisms of Huangqi in radiotherapy-induced kidney injury using network pharmacology. Materials and Methods: Active compounds of Huangqi were obtained from the TCMSP database and screened using oral bioavailability ≥30% and drug-likeness ≥0.18. Compound-related targets were standardized using UniProt and DrugBank. CKD-related targets were collected from GeneCards and DrugBank. Overlapping targets between Huangqi and CKD were identified as potential therapeutic targets. Protein-protein interaction analysis, GO enrichment, KEGG pathway enrichment, and compound-target-pathway network construction were performed using STRING, Cytoscape, and Metascape. Results: Twenty active compounds and 104 potential therapeutic targets were identified. Key targets included IL-6, TNF, AKT1, IL1B, TP53, MMP9, CASP3, PTGS2, JUN, and CXCL8. Enrichment analysis indicated that these targets were mainly involved in inflammatory response, apoptosis, oxidative stress, extracellular matrix remodeling, and cancer-related signaling pathways. These mechanisms are closely related to radiation-induced renal inflammation, fibrosis, and tissue injury. Conclusion: Huangqi may have potential mechanistic relevance in radiotherapy-induced kidney injury by regulating inflammation, apoptosis, oxidative stress, and fibrosis-related targets. These findings provide bioinformatics-based hypotheses for future experimental and clinical validation.</div> Astragalus membranaceus, chronic kidney disease, traditional Chinese medicine, network pharmacology, cancer, radiotherapy, molecular mechanism, radiation-induced nephropathy. 1157 1163 http://ijrr.com/browse.php?a_code=A-10-1-1628&slc_lang=en&sid=1 B. Zhang 7900319475328460035119 7900319475328460035119 No Xinyu Traditional Chinese Medicine Hospital, Xinyu, Jiangxi, China L. Zhuang 7900319475328460035120 7900319475328460035120 No Xinyu Traditional Chinese Medicine Hospital, Xinyu, Jiangxi, China W. Chen 7900319475328460035121 7900319475328460035121 No Xinyu Traditional Chinese Medicine Hospital, Xinyu, Jiangxi, China H. Ding 7900319475328460035122 7900319475328460035122 No Xinyu Traditional Chinese Medicine Hospital, Xinyu, Jiangxi, China X. Chen 125071658@qq.com 7900319475328460035123 7900319475328460035123 Yes Xinyu Traditional Chinese Medicine Hospital, Xinyu, Jiangxi, China