en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: People are exposed to more radiation than before with the application of radiation technology. Radiation is known to induce damage to cell structure, DNA, chromosomes and nucleus. In this study, we showed that CGJ extract can inhibit radiation-induced chromosomal damage in vivo and NLRP7 inflammasome activation in vitro, suggesting that the compound from CGJ can Be considered as a therapeutic materials to reduce adverse effect inflammation and chromosome aberration during radio-therapy. Materials and methods: In this study the inhibitory effects of Cheonggukjang (CGJ) extract on the radiation-induced micronucleus formation in vivo and the NOD-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome activation in vitro. Results: We observed the prolonged presence of radiation-induced inflammation and its onsecutive damage in hematopoietic tissues after irradiation by examining micronucleus formation in polychromatic erythrocytes. Mouse bone marrow-derived macrophages were used to investigate NLRP3 inflammasome activation. The micronucleus analysis with mouse peripheral polychromatic erythrocytes using acridine orange staining showed a significantly (p<0.05) reduced frequency of micronucleated bone marrow polychromatic erythrocytes (MnPCEs) in CGJ-treated mice. Conclusion: CGJ extract elevated the chance that mice would survive a potentially lethal dose of gamma-irradiation. Additionally, The CGJ extract attenuated IL-1&beta; maturation by interrupting the inflammasome. The CGJ extract attenuates radiation-induced micronucleus formation possibly mediated by inhibiting inflammasome, which provides valuable information for the further study of the mechanism of action of CGJ extract.</div> Cheonggukjang, radiation, micronuclei, inflammasome, NLRP3. 57 65 http://ijrr.com/browse.php?a_code=A-10-1-758&slc_lang=en&sid=1 J.H. Kim 7900319475328460012678 7900319475328460012678 No Department of Laboratory Animal Medicine, College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea L. Li 7900319475328460012679 7900319475328460012679 No Institute of Medical Science, Kangwon National University, Chuncheon, Republic of Korea D.H. Park 7900319475328460012680 7900319475328460012680 No Department of Oriental Medicine Material, Dongsin University, Republic of Korea I.W. Lee 7900319475328460012681 7900319475328460012681 No Department of Laboratory Animal Medicine, College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea N. Ham 7900319475328460012682 7900319475328460012682 No Department of Laboratory Animal Medicine, College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea H.S. Kim 7900319475328460012683 7900319475328460012683 No Radiation Health Research Institute, Korea Hydro and Nuclear Power Co.,Ltd. Seoul, Republic of Korea Y.J. Kim 7900319475328460012684 7900319475328460012684 No Korea Food Research Institute, Seongnam, Kyeonggi, Republic of Korea J. Shim 7900319475328460012685 7900319475328460012685 No Cancer Experimental Resources Branch, National Cancer Center, 809 Madu 1-dong, Goyang-si, Gyeonggi-do, 411-769, Republic of Korea S.W. Kwon 7900319475328460012686 7900319475328460012686 No CHA Bundang Medical Center, CHA University, Bundang-gu, Seongnam-si, Gyeonggi-do 463-712, Republic of Korea M.J. Lee mjlee@kangwon.ac.kr 7900319475328460012687 7900319475328460012687 Yes Department of Laboratory Animal Medicine, College of Veterinary Medicine Kangwon National University, Chuncheon, Republic of Korea