International Journal of Radiation Research

en Changes in the number and morphology of blood cells in mice pretreated with RNA preparations and exposed to 8 Gy of gamma radiation Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: In our recent studies, yeast double-stranded RNA showed radioprotective effect in mice exposed to 9.4 Gy of gamma radiation. The current work continues our inquiry and describes the changes in the number and morphology of blood cells in mice injected with double-stranded RNA from Saccharomyces cerevisiae prior to gamma irradiation. To be capable of estimating the required parameters, we have used 8 Gy of radiation, which allowed mice to survive for up to 37 days (LD60/30). Materials and Methods: Animals received single intravenous injections of one of the following compounds: (1) 7 mg of total RNA isolated from baking yeast Saccharomyces cerevisiae, (2) 200 μg of double-stranded RNA or (3) 400 μg of double-stranded RNA. 30 minutes later, using a 137Cs gamma emitter, mice were irradiated with a dose of 8 Gy at the rate of 1.4 Gy/min. The radioprotective effect of preparations was assessed based on death rates. Results: Injections of both 200 μg of double-stranded RNA and 7 mg of total RNA prior to irradiation provided the best radioprotective effects, ensuring the survival of 100% of animals. Changes in the number of blood cells and their morphological aberrations were being monitored for 37 days after irradiation. Conclusion: The protection from lethal radiation doses is associated with the preservation and rapid recovery of leukocytic and erythroid lineages.</div> Double-stranded RNA, lymphocytes, neutrophils, monocytes, mutagenic activity. 361 368 http://ijrr.com/browse.php?a_code=A-10-1-917&slc_lang=en&sid=1 T.D. Dubatolova 7900319475328460021592 7900319475328460021592 No Institute of Molecular and Cellular Biology, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia G.S. Ritter 7900319475328460021593 7900319475328460021593 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia A.S. Proskurina 7900319475328460021594 7900319475328460021594 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia P.E. Kisaretova 7900319475328460021595 7900319475328460021595 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia V.P. Nikolin 7900319475328460021596 7900319475328460021596 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia N.A. Popova 7900319475328460021597 7900319475328460021597 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia V.S. Ruzanova 7900319475328460021598 7900319475328460021598 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia O.S. Taranov 7900319475328460021599 7900319475328460021599 No State Research Center of Virology and Biotechnology “Vector”, Koltsovo, Novosibirsk region, Russia N.A. Kolchanov 7900319475328460021600 7900319475328460021600 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia S.S. Bogachev 7900319475328460021601 7900319475328460021601 No Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia 3Novosibirsk State University, Novosibirsk, Russia