International Journal of Radiation Research

en Effects of timing in the applications of radiotherapy after transverse rectus abdominis musculocutaneous flap in rats Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: The present study aimed to investigate flap status in relation to the timing of radiotherapy (RT) in an experimental rat model of irradiated transverse rectus abdominis musculocutaneous (TRAM) flap. Materials and Methods: Fifty-six adult male Sprague-Dawley experiments were separated in seven groups in terms of flap surgery and RT. The groups comprised sham group, control (Ctrl) group, RT group, and RT plus surgery (RT+Surg) group, which was further separated in four groups depending on the timing of postoperative RT as RT+Surg-10 (RT on postoperative 10th day), RT+Surg-20 (RT on postoperative 20th day), RT+Surg-30 (RT on postoperative 30th day), and RT+Surg-40 (RT on postoperative 40th day). All the rats were sacrificed 8 weeks after the RT administration for histopathological analysis. Results: Compared with the RT+Surg-10 and RT+Surg-20 groups, the hyalinization and collagenization scores in RT+Surg-30 were determined to be significantly lower. Fibrosis scores were lower in the RT+Surg-30 group compared with the RT+Surg-20 group, whereas significantly lower inflammation scores were determined in the RT + Surg-40 group and significantly higher dermal thickness in the RT+Surg-30 group compared with the RT+Surg-10 group. Conclusions: The findings from this model of irradiated flap revealed the significance of the time lag among flap application and postoperative RT for histopathological outcome, emphasized the potential role of at least a 30- to 40-day interval between surgery and RT in achieving more favorable flap status.</div> TRAM flap, rat, timing of irradiation, experimental, histopathological outcomes. 649 655 http://ijrr.com/browse.php?a_code=A-10-1-959&slc_lang=en&sid=1 O. Binicier 7900319475328460022132 7900319475328460022132 No Private Clinic, Bursa, Turkey P.K. Caskan 7900319475328460022133 7900319475328460022133 No University of Health Sciences, Sisli Hamidiye Etfal Training and Research Hospital, Department of Plastic, Reconstructive and Aesthetic Surgery, Istanbul, Turkey E. Kapi eminkapi@gmail.com 7900319475328460022134 7900319475328460022134 Yes University of Health Sciences, Adana Faculty of Medicine, Health Application and Research Center, Department of Plastic, Reconstructive and Aesthetic Surgery, Adana, Turkey M. Bozkurt 7900319475328460022135 7900319475328460022135 No University of Health Sciences, Bagcilar Health Application and Research Center, Department of Plastic, Reconstructive and Aesthetic Surgery, Istanbul, Turkey S.B. Zincircioğlu 7900319475328460022136 7900319475328460022136 No Dicle University, Faculty of Medicine, Department of Radiation Oncology, Diyarbakir, Turkey 6University of Pittsburgh, Little Lab, Pittsburgh, Pennsylvania, USA Y. Kulahcı 7900319475328460022137 7900319475328460022137 No University of Pittsburgh, Little Lab, Pittsburgh, Pennsylvania, USA C.T. Selcuk 7900319475328460022138 7900319475328460022138 No Dicle University, Faculty of Medicine, Department of Plastic, Reconstructive an Aesthetic Surgery, Diyarbakir, Turkey O. Satici 7900319475328460022139 7900319475328460022139 No Dicle University, Faculty of Medicine, Department of Biostatistics, Diyarbakir, Turkey