en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Radiotherapy is an important and effective modality in treating cancer patients. Radiation-induced cardiovascular injury is one of the consequences of radiotherapy due to undesired exposure of the heart. The non-targeted effects of radiation add to the risk of cardiovascular injury following radiotherapy. Accordingly, we investigated the abscopal effect of radiation in the myocardium of partially irradiated rats and compared the abscopal effect with the effect induced in the myocardium following either direct or sham irradiation. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Twenty male rats divided into four groups; sham, 2Gy whole-body, 2Gy cranially- and 2Gy lower-limb irradiated, were used. Myocardium samples were collected and examined for MDA, p53, caspase 3, and glutathione reduced expression. Additionally, TGF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&beta; </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">and NF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&kappa;</span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">B expression levels were investigated. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Our data revealed elevated levels of lipid peroxidation in abscopal myocardium following cranial- /lower-limb irradiation with 2 Gy </span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&gamma;-</span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">radiation, as compared with the sham or whole-body irradiated groups. Similar behavior was noticed with p53 and caspase3 expression, suggesting apoptosis induction in the abscopal cardiomyocytes. The rise in lipid peroxidation and apoptosis markers in the abscopal myocardium was associated with an increase in glutathione reduced. The data propose oxidative stress induction in the myocardium following distant irradiation, which was further confirmed by the elevation in TGF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&beta; </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">and nuclear NF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&kappa;</span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">B expression. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Abscopal effect was induced in the myocardium of partially-irradiated rats, suggesting increased risk of myocardial injury following localized radiotherapy.</span></span></span></span></span></span></span></span></span></div> Ionizing radiation, abscopal effect, lipid peroxidation, oxidative stress, cytokines. 289 295 http://ijrr.com/browse.php?a_code=A-10-1-1172&slc_lang=en&sid=1 L.M. Ramadan 7900319475328460026776 7900319475328460026776 No Biochemistry Department, Biotechnology Research Institute, National Research Centre, Cairo, Egypt. 12622 A.B. Abdelrazzak a.b.abdelrazzak@gmail.com 7900319475328460026777 7900319475328460026777 Yes Spectroscopy Department, Physics Research Institute, National Research Centre, Cairo, Egypt. 12622