en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The present study aimed to evaluate the effect of gamma-irradiation (5kGy) on total phenolic and total flavonoid content, antioxidant capacity, and concentration of phenolic compounds of olive leaves, and to assess the potential hypoglycemic effect of gamma-irradiated olive leaf extract (GOLE) in alloxan-induced diabetic rats. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The results indicated that gamma-irradiation caused a significant increase in the total phenol content, total flavonoids, and antioxidant activity in olive leaves compared to raw leaves. High-Performance Liquid Chromatography (HPLC) analysis showed that gamma-irradiation increased the values of phenolic compounds in olive leaves such as hydroxytyrosol, hydroxytyrosol-1-glucoside, rutinhydrate, and tyrosol. The results of the biological study showed that the co-administration of alloxan (150 mg/kg body weight) together with either raw (ROLE) or gamma-irradiated olive leaf extract (GOLE) (1ml/100 gm /body weight/ day/ 8 weeks) to rats caused hypoglycemia, hyperinsulinemia, decreased level of the homeostatic index of insulin resistance (HOMA-IR), </span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&alpha;-</span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">amylase and </span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&alpha;-</span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">glucosidase activity, and a significant elevation in hepatic glycogen storage and the activity of hepatic hexokinase, glucose-6-phosphate dehydrogenase, pyruvate kinase and glycogen synthase associated with a significant reduction in hepatic glucose 6-phosphatase, fructose 1,6-bisphosphatase, glycogen phosphorylase, the activity of liver marker enzymes and decreased level of serum creatinine, uric acid, and urea compared to the diabetic group. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The results also showed that the anti-diabetic effect of GOLE was more significant than that observed with ROLE because of the effectiveness of gamma-irradiation (5 kGy) in improving the antioxidant activity and increasing the concentration of biological components of olive Leaf.</span></span></span></span></span></span></span></span></span></div> Hypoglycemic effect, alloxan, gamma-irradiation, olive leaf extract, phenolic compounds, antioxidant activity. 845 851 http://ijrr.com/browse.php?a_code=A-10-1-1248&slc_lang=en&sid=1 M.H.M. Abd el Megid mohamed.h.m.abd.elmegid@gmail.com 7900319475328460028392 7900319475328460028392 Yes Natural Products Department, Food Irradiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt A.M. Abdul Azeem 7900319475328460028393 7900319475328460028393 No Natural Products Department, Food Irradiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt A.N. El-Shahat 7900319475328460028394 7900319475328460028394 No Natural Products Department, Food Irradiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt A.M. Mounir 7900319475328460028395 7900319475328460028395 No Natural Products Department, Food Irradiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt