en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">DNA methyltransferase 2 (DNMT2) is reported as an RNA modifier regulating the expression of oncogenes in cancers. This work explored the regulatory potential of DNMT2 in gastric cancer (GC) cell proliferation and migration. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">GC cells were induced with Transforming growth factor-beta1 (TGF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&beta;1) </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">for constructing DNMT2 overexpression model. GC cell proliferation was subject to Cell Counting Kit-8 (CCK-8) assay. GC cell migration was observed through wound scratch together with transwell migration assays. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The outcomes showed that the DNMT2 overexpression model was successfully built. Relative to the control, the levels of DNMT2 and SMC3 were apparently decreased by TGF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&beta;1 </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">stimulation, whereas E-cadherin, Smad2 as well as Vimentin expression was elevated by TGF-</span></span></span><span lang="el" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:el">&beta;1, </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">and GC cell proliferation along with migration were significantly elevated. However, in comparison with the NC group, the DNMT2 overexpression group exhibited higher levels of DNMT2 and SMC3, significantly suppressed E-cadherin, Vimentin along with Smad2 expression, and significantly suppressed GC cell proliferation along with migration. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Overexpression of DNMT2 inhibits GC cell proliferation along with migration. The level of SMC3 was also elevated by DNMT2 overexpression in GC cells. The findings of our study might provide the theoretical basis for the development of GC.</span></span></span></span></span></span></span></span></span></div> DNMT2, SMC3, gastric cancer. 897 902 http://ijrr.com/browse.php?a_code=A-10-1-1255&slc_lang=en&sid=1 T.F. Xia jsxiatianfang@163.com 7900319475328460028502 7900319475328460028502 Yes Department of Gastroenterology, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, China Z.J. Zhang 7900319475328460028503 7900319475328460028503 No Department of Oncology, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, China X. Guo 7900319475328460028504 7900319475328460028504 No Department of Gastroenterology, The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University, Huai’an, Jiangsu 223300, China