International Journal of Radiation Research

en Cytogenetic and cytotoxic effects of melatonin and saffron on lymphocytes of luminal A and luminal B breast cancer patients irradiated in vitro Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;">Background: Due to high radiation- sensitivity of breast cancer (BC) patients, use of radioprotectors to ameliorate the deleterious effects of radiation could be a priority. This study aimed to evaluate the reducing effects of saffron and melatonin on ionizing radiation-induced damages in lymphocytes of luminal A & B BC patients using cytome assay. Materials and Methods: Whole blood samples were collected from BC patients as well as healthy individuals. Cells were treated with either melatonin or saffron two hours prior to irradiation of G0 lymphocytes using a 6 MV linear accelerator, at a dose of 3Gy. Thirty-six hours after PHA stimulation, Cytochalasin-B was added to the cultures. Cell harvesting and staining was performed using standard method. 1000 binucleate cells were scored per sample for the frequency of micronuclei (MN) or apoptosis under a 1000x light microscope. Results: The frequency of background MN was significantly lower in normal individuals compared to BC patients. Pretreatments of "saffron and melatonin" alone made no significant changes in the frequency of MN in all groups. However, after 3Gy-irradiation the frequency of MN elevated dramatically (p<0.01). Pretreatment of lymphocytes with melatonin and saffron led to a decrease in the frequency of MN in all treatment groups (p<0.05). In terms of apoptosis induction, only melatonin was found to exert reducing effect on radiation induced apoptosis. No radioprotection was observed for saffron in lymphocytes of BC patients. Conclusion: Obtained results elucidated more pronounced role of melatonin in decreasing MN and apoptosis frequencies post irradiation in lymphocytes of BC patients.</div> Cytome assay, breast cancer, lymphocytes, antioxidants, ionizing radiation, radioprotection. 37 43 http://ijrr.com/browse.php?a_code=A-10-1-1289&slc_lang=en&sid=1 H. Mozdarani mozdarani@modares.ac.ir 7900319475328460029966 7900319475328460029966 Yes Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran S. Mozdarani 7900319475328460029967 7900319475328460029967 No Department of Cytogenetics, Cytogenome Medical Genetics laboratory, Ale-Ahmad High way, Chamran Medical Building, Tehran, Iran F. Pakniyat 7900319475328460029968 7900319475328460029968 No Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran S. Mozdarani 7900319475328460029969 7900319475328460029969 No Department of Cytogenetics, Cytogenome Medical Genetics laboratory, Ale-Ahmad High way, Chamran Medical Building, Tehran, Iran H. Nosrati 7900319475328460029970 7900319475328460029970 No Cancer Institute, Department of Radiotherapy, Tehran, Iran