en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-weight:bold"><span style="language:en-US">:</span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The aim of this study was to investigate the effects of ozone and melatonin interventions on anti-oxidant effects and enzymatic parameters in the liver and kidneys of mice experiencing busulfan-induced injury, a common chemotherapy-related condition known to severely affect these organs. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods:</span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US"> The research included 24 male mice were randomly assigned in four groups of six mice each: a control group without any intervention, busulfan group, busulfan with melatonin group, and busulfan with ozone group. This experimental setup spanned seven days. After a 35-day period, blood, liver and kidney tissues were extracted for the assessment of various parameters, such as super oxide dismutase (SOD), catalase, total antioxidant capacity (TAC), malondialdehyde, and tissue creatinine. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Busulfan administration resulted in a decrease in SOD, catalase, and TAC levels, along with an increase in malondialdehyde levels in both the kidneys and liver. Additionally, Busulfan led to elevated ALT levels and decreased AST in liver tissue, as well as increased BUN and tissue creatinine in the kidneys. However, the introduction of ozone and melatonin ameliorated these effects across all parameters. Notably, ozone exhibited a stronger impact on catalase, TAC, and malondialdehyde in both organs. Moreover, ozone showed pronounced effects on ALT and AST in the liver and on creatinine in the kidneys. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Both melatonin and ozone show promise in ameliorating Busulfan-induced injury. Interestingly, ozone appears to exhibit greater efficacy in enhancing kidney and liver function in such cases.</span></span></span></span></span></span></span></span></span></div> Busulfan, melatonin, ozone, oxidative stress, liver, kidney. 349 355 http://ijrr.com/browse.php?a_code=A-10-1-1336&slc_lang=en&sid=1 R. Dadfar 7900319475328460031076 7900319475328460031076 No Department of Anatomical Sciences, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran T. Safari 7900319475328460031077 7900319475328460031077 No Department of Histology, Faculty of Veterinary Medicine Department of Basic Sciences, Shahid Chamran University of Ahvaz, Iran M. Taheri Moghadam taherimoghadam-m@ajums.ac.ir 7900319475328460031078 7900319475328460031078 Yes Department of Anatomical Sciences, Faculty of Medicine, Ahvaz Jundishapur University of Medical sciences, Ahvaz, Iran