en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Colon cancer (CC) is one of the most common malignancies in the digestive tract. In this study, we analysed the diagnostic value of multislice CT (MSCT) in combination with miR-let-7 in CC and the mechanism of action of miR-let-7 in CC. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">A total of 108 patients with suspected CC admitted to our hospital between March 2020 and May 2022 were retrospectively analysed; 37 patients were diagnosed with CC, and the remaining 71 were determined to have benign colonic lesions. To compare the diagnostic value of MSCT, miR-let-7, and MSCT combined with miR-let-7 detection for CC, we detected the expression of miR-let-7 and high mobility group AT hook protein 2 (HMGA2) in normal human colonic epithelial FHC cells and human colonic mucosal epithelial HCT116 cells and observed the effect of miR-let-7 on the biological behaviour of CC cells. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Compared with the results of pathological biopsy, the diagnostic sensitivity of MSCT combined with miR-let-7 for CC was 97.30%, the specificity was 98.59%, and the accuracy was 98.15%, with a misdiagnosis rate of only 1.85%. After miR-let-7 expression was silenced, the viability of colon cancer cells increased, and the inflammatory response and oxidative stress increased. In addition, there was a targeted regulatory relationship between HMGA2 and miR-let-7, which could reverse the effect of miR-let-7 on colon cancer cells. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The combination of MSCT and the miR-let-7 assay is accurate for the diagnosis of CC, and miR-let-7 regulates the biological behaviour of CC cells through HMGA2.</span></span></span></span></span></span></span></span></span></div> Colonic neoplasms, HMGA2 protein, multidetector computed tomography, inflammation, miR-let-7. 569 576 http://ijrr.com/browse.php?a_code=A-10-1-1369&slc_lang=en&sid=1 K. Yang 7900319475328460031366 7900319475328460031366 No Z. Chen wolf0330cz.edu@hotmail.com 7900319475328460031367 7900319475328460031367 Yes Department of General Surgery, The Affiliated Changsha Hospital of Xiangya School of Medicine, Central South University, Changsha, China