en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">This research attempted to elucidate amide proton transfer (APT) role in combination with relative cerebral blood volume (rCBV) in grading adult diffuse glioma and predicting isocitrate dehydrogenase (IDH) gene mutation status. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">A retrospective analysis was implemented on 70 patients with adult diffuse glioma in our hospital from September 2022 to October 2023. All patients underwent Dynamic Susceptibility Contrast Perfusion Weighted Imaging (DSC-PWI) and APT-weighted magnetic resonance imaging examination. The values of rCBVmax and APTmean in differentiating the classification of glioma and IDH gene mutation status were analyzed. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The rCBVmax and APTmean value were reduced in grade II patients than in grade III patients (P<0.01). The AUC of rCBVmax value combined with APTmean value was 0.9330, with a 95% CI of 0.8962-0.9697, which was higher than single rCBVmax value or APTmean value (P<0.001). The rCBVmax value and APTmean were higher in IDH wild-type glioma patients than in IDH mutant-type glioma patients (P<0.01). The AUC of rCBVmax value combined with APTmean value was 0.8808, with a 95% CI of 0.8233-0.9383, which was higher than single rCBVmax value or APTmean value (P<0.001). </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The combined diagnosis of APT and rCBV can improve the value of differentiating grade I and II glioma as well as IDH genotyping, which is worth of promoting in clinical practice.</span></span></span></span></span></span></span></span></span></div> Glioma, grade, isocitrate dehydrogenase, gene. 737 742 http://ijrr.com/browse.php?a_code=A-10-1-1392&slc_lang=en&sid=1 S. Chen 7900319475328460032056 7900319475328460032056 No Department of Radiology, Quanzhou First Hospital, Quanzhou, Fujian, China R. Huang hrisheng@126.com 7900319475328460032057 7900319475328460032057 Yes Department of Radiology, Quanzhou First Hospital, Quanzhou, Fujian, China J. Chen 7900319475328460032058 7900319475328460032058 No Department of Radiology, Quanzhou First Hospital, Quanzhou, Fujian, China H. Lin 7900319475328460032059 7900319475328460032059 No Department of Radiology, Quanzhou First Hospital, Quanzhou, Fujian, China L. Lu 7900319475328460032060 7900319475328460032060 No Department of Radiotherapy, Quanzhou First Hospital, Quanzhou, Fujian, China S. Zhang 7900319475328460032061 7900319475328460032061 No Department of Radiology, Quanzhou First Hospital, Quanzhou, Fujian, China Z. Jiang 7900319475328460032062 7900319475328460032062 No Department of Neurosurgery, Quanzhou First Hospital, Quanzhou, Fujian, China