en Radiation Biology Radiation Biology تحقيق بديع Original Research <div style="text-align: justify;"><span style="font-size:10pt"><span style="text-justify:newspaper"><span style="text-kashida-space:50%"><span style="line-height:119%"><span style="font-family:Calibri"><span style="color:black"><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Background:</span></span></span></span></span></span> <span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">As the predominant malignant embryonal liver tumor in children, hepatoblastoma (HB) demonstrates rising incidence rates. While surgical resection and chemotherapy remain primary treatments, radiation therapy emerges as a potential adjuvant option for refractory cases. However, clinical evidence supporting its application remains scarce. To address this knowledge gap, we investigated the radiobiological response of HB cell line Huh-6 through systematic </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="font-style:italic"><span style="language:en-US">in vitro </span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">analyses. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Materials and Methods: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">The proliferation, morphology change, cell apoptosis and cell cycle of Huh-6 cells received gradient X-ray irradiation were systemically conducted with CCK-8 assay, microscopy, Annexin V/7-ADD and PI single staining, respectively. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Results: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Irradiation induced dose-dependent suppression of Huh-6 cell proliferation. Morphological assessment demonstrated escalating cytopathic effects with increasing radiation doses, characterized by cytoplasmic vacuolization in the early stage progressing to membrane disintegration and cytosolic debris formation in morphologically aberrant cells during the late stage. Cell apoptosis was enhanced with the increment of irradiation dose. The percentage of cells arrested in G2/M phase increased and meanwhile the proportion of cells in G0/G1 phase decreased with the rising dose gradient. </span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="color:#1f497d"><span style="font-style:italic"><span style="font-weight:bold"><span style="language:en-US">Conclusion: </span></span></span></span></span></span><span lang="en-US" style="font-size:9.0pt"><span style="font-family:Calibri"><span style="language:en-US">Our findings demonstrate marked radiosensitivity in Huh-6 cells characterized by dose-responsive growth inhibition, cell apoptosis and cell morphological changes. These experimental results provide preliminary biological validation for considering radiotherapy in HB management protocols, particularly for treatment-resistant cases.</span></span></span></span></span></span></span></span></span></div> Hepatoblastoma, radiosensitivity, Huh-6 cell line, in vitro. 895 900 http://ijrr.com/browse.php?a_code=A-10-1-1412&slc_lang=en&sid=1 L. Xu 7900319475328460032331 7900319475328460032331 No Cancer Research Center Nantong, Affiliated Tumor Hospital of Nantong University, and Medical School of Nantong University, Nantong, China Q. Ge geqin.kelley@163.com 7900319475328460032332 7900319475328460032332 No Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University, Nantong, China