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:: Volume 22, Issue 1 (1-2024) ::
Int J Radiat Res 2024, 22(1): 91-95 Back to browse issues page
The effect of radiation-induced genotoxic stress on the expression of satellite II and satellite III repeats in breast and colon cancer cells
E. Yilmaz , E. Ozgur , S.B.Gazioglu Gazioglu , C.K. Akbas , U. Gezer , E.E. Yoruker
Department of Basic Oncology, Institute of Oncology, Istanbul University, 34093 Istanbul, Turkey , akisik@istanbul.edu.tr
Abstract:   (662 Views)
Background: Pericentric human satellite II (HSATII) and III (HSATIII) have been shown to be associated with stress response. Expression status of these satellite repeats has not yet been investigated under radiation-induced genotoxic stress. We evaluated the HSATII and HSATIII expression changes under genotoxic stress in cancer cells. Materials and Methods: Cell line MCF-7 and cell line HCT-15 were irradiated with 2 and 5 Gy of ionizing radiation, and cell death rates, as a consequence of genotoxic stress, were determined by flow cytometry. The expression of HSATII and HSATIII expressions was assessed by RT-qPCR. Results: Radiation exposure induced a considerable level of cell death in a dose-dependent manner in both cell types. Compared to untreated cells, HSATII expression declined in MCF-7 cells which were exposed to 2 and 5 Gy radiation, respectively. In HCT-15 cells, the effect of radiation on the HSATII expression was not unified; only higher radiation dose led to a decrease of HSATII expression while 2 Gy increased HSATII expression. The effect of radiation on the HSATIII repeat expression was more pronounced in HCT-15 cells: in MCF-7 cells, HSATIII expression was decreased by 2- and 5-Gy, respectively (p=0.01). In the HCT-15 cells, the rates of HSATIII down-regulation were 3-fold and 2.8 fold by 2- and 5 Gy (p=0.0002 and p=0.02, respectively). Conclusion: Our findings reveal that genotoxic stress induced by ionizing radiation is associated with a decrease in the expression of pericentric satellites and the expression status of HSATII in these conditions may be dose- and/or cellular context-specific.
Keywords: Satellite DNA, breast cancer, colon cancer, radiation.
Full-Text [PDF 663 kb]   (358 Downloads)    
Type of Study: Original Research | Subject: Radiation Biology
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Yilmaz E, Ozgur E, Gazioglu S, Akbas C, Gezer U, Yoruker E. The effect of radiation-induced genotoxic stress on the expression of satellite II and satellite III repeats in breast and colon cancer cells. Int J Radiat Res 2024; 22 (1) :91-95
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Volume 22, Issue 1 (1-2024) Back to browse issues page
International Journal of Radiation Research
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