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The potential hypoglycemic effect of aqueous extract of gamma-irradiated olive leaves in alloxan-induced diabetic rats
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M.H.M. Abd el Megid   , A.M. Abdul Azeem , A.N. El-Shahat , A.M. Mounir |
| Natural Products Department, Food Irradiation Research Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority (EAEA), Cairo, Egypt , mohamed.h.m.abd.elmegid@gmail.com |
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Abstract: (48 Views) |
Background: The present study aimed to evaluate the effect of gamma-irradiation (5kGy) on total phenolic and total flavonoid content, antioxidant capacity, and concentration of phenolic compounds of olive leaves, and to assess the potential hypoglycemic effect of gamma-irradiated olive leaf extract (GOLE) in alloxan-induced diabetic rats. Results: The results indicated that gamma-irradiation caused a significant increase in the total phenol content, total flavonoids, and antioxidant activity in olive leaves compared to raw leaves. High-Performance Liquid Chromatography (HPLC) analysis showed that gamma-irradiation increased the values of phenolic compounds in olive leaves such as hydroxytyrosol, hydroxytyrosol-1-glucoside, rutinhydrate, and tyrosol. The results of the biological study showed that the co-administration of alloxan (150 mg/kg body weight) together with either raw (ROLE) or gamma-irradiated olive leaf extract (GOLE) (1ml/100 gm /body weight/ day/ 8 weeks) to rats caused hypoglycemia, hyperinsulinemia, decreased level of the homeostatic index of insulin resistance (HOMA-IR), α-amylase and α-glucosidase activity, and a significant elevation in hepatic glycogen storage and the activity of hepatic hexokinase, glucose-6-phosphate dehydrogenase, pyruvate kinase and glycogen synthase associated with a significant reduction in hepatic glucose 6-phosphatase, fructose 1,6-bisphosphatase, glycogen phosphorylase, the activity of liver marker enzymes and decreased level of serum creatinine, uric acid, and urea compared to the diabetic group. Conclusion: The results also showed that the anti-diabetic effect of GOLE was more significant than that observed with ROLE because of the effectiveness of gamma-irradiation (5 kGy) in improving the antioxidant activity and increasing the concentration of biological components of olive Leaf. |
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| Keywords: Hypoglycemic effect, alloxan, gamma-irradiation, olive leaf extract, phenolic compounds, antioxidant activity. |
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Full-Text [PDF 619 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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