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Umbelliferone as a promising protective agent in rat kidney against high dose post-irradiation injury; biochemical, immunohistochemical and histopathological evaluations
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F. Geyikoglu   , G.Y. Deniz , M.N.G. Demir , M. Arslan , B. Erdemci |
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Abstract: (40 Views) |
Background: Irradiation at the high dose may attenuate renal hemodynamics while retaining the therapeutic effect on cancer cells and thus can life-threatening. Hence the effects on kidneys of irradiation become increasingly crucial. This study aimed to examine oxidative stress, inflammatory response, vascular injury, apoptotis and deoxyribonucleic acid (DNA)DNA damage induced acute high dose ionising radiation in rat kidney and to investigate whether umbelliferone (UMB) has protective effect on radiation-induced renal damage and dysfunction. Materials and Methods: Rats were randomly allocated into eight groups (n = 7): the control group, given physiological saline; the model group, exposured radiation (whole body irradiated with single high dose of 12 Gy of gamma rays) to induce radiation damage in renal tissue; UMB groups, received UMB (30, 60 and 90 mg/kg) alone and treatment groupsreceived orally UMB for 5 days before radiation exposure. Animals were divided groups; control, group whole body irradiated with single dose 12 Gy, groups receiving umbelliferone alone and groups receiving umbelliferone for 5 days before irradiated. Results: Radiation-induced pathological findings and functional disorders were significantly ameliorated in UMBumbelliferone-treated groups. Our results revealed that umbelliferone pretreatment in the kidney plays an important role in the regulation of blood pressure in irradiation-induced renal injury. UMB increases the antioxidant activity and decreases inflammatory response in kidney tissue against radiation toxicity. Notably, it exhibits anti-apoptotic and anti-genotoxic actions in damaged renal cells. It also ameliorates the blood urea nitrogen (BUN) and creatinine values. Conclusion: In conclusion, UMBumbelliferone from coumarin compounds is a targeted and promising drug to prevent kidney necrosis and dysfunction due to radiation therapy. |
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| Keywords: 8-hydroxy-2'-deoxyguanosine (8-OHdG)DNA damage, ıonizing Rradiation, renal dysfunctioninjury, umbelliferone, thromboxane B2 (TXB2)Vascular injury. |
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Full-Text [PDF 1817 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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