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The value of Micro-CT in the detection of nicotine induced alveolar bone osteogenesis and microstructure in rats
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X. Li   , J. Mi , Y. Zhang |
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Abstract: (461 Views) |
Background: Explore the effects of different concentrations of nicotine on the microstructure of rat alveolar bone and the expression of osteogenic related proteins, in order to clarify its role in alveolar bone osteogenic metabolism. Materials and Methods: In this study, 28 8-week-old male Sprague Dawley (SD) rats were randomly divided into four groups (control group, low-dose group, medium dose group, high-dose group) and subjected to continuous intraperitoneal injection intervention for 180 days. Subsequently, the microstructure characteristics of alveolar bone were analyzed using micro computed tomography (Micro-CT), and the expression of osteogenic related proteins was accurately determined by combining immunohistochemistry and qRT PCR techniques. Results: Nicotine dose dependently reduces the volume of rat alveolar bone and damages its microstructure; Nicotine dose dependently reduces the expression of type I collagen (COL-I), alkaline phosphatase (ALP), osteocalcin (OCN), and bone sialoprotein (BSP), while increasing the expression of osteopontin (OPN). Conclusion: Nicotine interferes with the osteogenic process of rat alveolar bone by affecting the expression of osteogenic related proteins. |
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| Keywords: Nicotine, alveolar bone, osteogenesis-related proteins, microstructure. |
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Full-Text [PDF 1026 kb]
(152 Downloads)
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Type of Study: Original Research |
Subject:
Radiation Biology
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