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Ganoderma lucidum constituents suppress A431 cell proliferation and TGF-β/Smad-related gene expression in cutaneous squamous cell carcinoma
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Y. Zhang   , W. Xiong , P. Hao , G. Zhang , Y. Wang , R. Gao , H. Tan |
| Department of Dermatology, People's Hospital Affiliated to Chongqing Three Gorges Medical College, Chongqing, China , sintist@163.com |
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Abstract: (28 Views) |
Background: To investigate the combined effects of Ganoderma lucidum (Ganoderic acid B and Ganoderma polysaccharides) and radiation on the proliferation and gene expression of skin squamous cell carcinoma (SCC) A431 cells, focusing on the TGF-β/Smad signaling pathway. Materials and Methods: A431 cells were treated with varying concentrations of Ganoderic acid B (0–400 µmol/L) and Ganoderma polysaccharides (0–10 mg/mL), either alone or in combination with radiation doses (0, 2, 4, 8 Gy). Cell viability was assessed using the CCK-8 assay after 24 hours. The mRNA expression levels of TGF-β1, GDF11, Smad2, Smad4, and Smad7 were analyzed via quantitative real-time PCR. Results: Both Ganoderic acid B and Ganoderma polysaccharides inhibited A431 cell proliferation in a concentration-dependent manner. Radiation alone also reduced cell viability. The combined treatment of Ganoderma with radiation exhibited a synergistic inhibitory effect, significantly enhancing cytotoxicity compared to monotherapies (p < 0.05). Ganoderic acid B and Ganoderma polysaccharides reduced the expression of TGF-β/Smad-related genes. The potential interaction with radiation requires further investigation. Conclusion: Ganoderma lucidum enhances the radiosensitivity of skin SCC cells, potentially through cooperative suppression of the TGF-β/Smad signaling pathway. These findings suggest a promising integrative strategy to improve the efficacy of radiotherapy in the treatment of skin SCC. |
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| Keywords: Carcinoma, squamous cell, ganoderma lucidum, radiotherapy, radiosensitivity, transforming growth factor beta, SMAD proteins. |
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Full-Text [PDF 976 kb]
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Type of Study: Original Research |
Subject:
Radiation Biology
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